Crosslinking, Labeling and Protein Modification
Cayman Chemical Biotin-azide, Purity: ≥98%, CAS: 908007-17-0, 25 mg
A form of biotin with a terminal azide group; is used to add biotin to other molecules that bear either an alkyne group, through click chemistry, or a phosphine group, using Staudinger ligation
Cayman Chemical Biotin-XX hydrazide, Purity: ≥95%, CAS: 211237-33-1, 5 mg
A biotinylating reagent that contains two aminohexanoic spacers to increase the efficiency of avidin-binding by alleviating steric hindrance
Cayman Chemical Propidium Iodide, Purity: ≥90%, CAS: 25535-16-4, 10 mg
A fluorescent probe commonly used to identify dead cells; excitation at 488-535 nm and emission maximum of 617 nm
Cayman Chemical PhosphIn-bIotIn 500ug
A labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction; can be used in common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE; has been used successfully in conjunction with Daz-1 or Daz-2 to label and detect sulfenic acid sites in proteins
Cayman Chemical Myristic Acid, Purity: ≥98%, CAS: 544-63-8, 5 g
A saturated fatty acid; incorporated into myristoyl-CoA and transferred by N-myristoyltransferase to the N-terminal glycine of certain proteins
Sigma Aldrich Fine Chemicals Biosciences CfTM 647 succinimidyl este
Succinimidyl esters are amine reactive fluorescent dyes which function as probes in arrays studies and are used to label proteins.
Sigma Aldrich Fine Chemicals Biosciences Collagenase B from Clostri
Collagenase B is prepared from Clostridium histolyticum cultures by filtration ammonium sulfate precipitation dialysis and lyophilization. Collagenase from Clostridium histolyticum is a collagenolytic enzyme. It interacts with collagen-like substances and is responsible for the degradation of collagen into small peptides. It is widely used for the disaggregation of many types of tissues (e.g. lung heart muscle bone adipose tissue liver kidney cartilage mammary gland placentae blood vessels brain tumors) and for the preparation of single-cell suspensions for the establishment of primary cell culture systems. Collagenase B is recommended when yield and viability are important.
